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To generate myofibroblast cultures, HCFs have been seeded employing DMEM that contains ten% serum, after 8 h switched to serumfree medium containing TGFb1 (five ng/ml)

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Immunofluorescence staining for aSMA (myofibroblast marker) fibronectin (tissue transforming marker) and osteoclacin (osteoblast marker)...
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These data stand in sharp distinction to estimates of survival generated a 10 years in the past in which, in the period of cytokine therapies, a median survival approaching 1 year was predicted [eighteen]

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Reporting of tumor histology, quality and stage was performed in accordance with the Worldwide...
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The protein concentrations of supernatants immediately after centrifugation (twelve,000 g, 5 min, 4uC) have been measured with a Pierce BCA assay kit (Thermo Scientific, Scoresby, Victoria, Australia) in accordance to manufacturer’s guidance

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Unaffected South Hampshire and Merino sheep, and CLN5 (Borderdale) heterozygous (CLN5+/2, unaffected) animals ...