To take a look at exactly where CKIP-one localizes in Jurkat T cells, the detergent-insoluble membrane (lipid raft) fractions ended up ready by the extremely-centrifugation in a discontinuous OptiPrep density gradient. Lck was constitutively related with lipid rafts, and PKCh was recruited
The genes rendering the reversion of the mutant phenotype ended up isolated by PCR...