The protein concentrations of supernatants immediately after centrifugation (twelve,000 g, 5 min, 4uC) have been measured with a Pierce BCA assay kit (Thermo Scientific, Scoresby, Victoria, Australia) in accordance to manufacturer’s guidance
Unaffected South Hampshire and Merino sheep, and CLN5 (Borderdale) heterozygous (CLN5+/2, unaffected) animals ...