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TNF-R1 (human) monoclonal antibody (H398)
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| Alternative Name TNF-R 60kDa, CD120a, p55, TNFRSF 1A | Application Flow Cytometry, FUNC, IHC (FS) | Application Notes Not recommended for IHC (PS).Functional Application: blocks TNF-R signalling. 10µg/ml H398 neutralizes TNF induced cytotoxicity on U937 (100%). Furthermore, 10µg/ml H398 neutralizes TNF induced MHC expression on K562, Colo 205 and IL2R on YT cells (always 100%). TNF induced cytotoxicity can be inhibited/neutralized by H398 on MCF-7, HeLa and L929. A working concentration could not be determined, since ascites antibody preparations have been used which exhibit an unspecific cytotoxic effect on the cells.128446-35-5 supplier Functional activity via the TNF-R1 is completely inhibited by H398 although on some cells the ligand still binds. Thus, the antibody H398 inhibits signalling via the receptor but not always the binding of TNF. | Clone H398 | Crossreactivity Does not cross-react with mouse TNF-R1. | Formulation Liquid. In PBS.872573-93-8 web | Host Mouse | Immunogen Human TNF-R1.PMID:20301469 | Isotype IgG2a | Recommendation Dilutions/Conditions Immunohistochemistry (frozen sections; stains acetone-fixed cryostat sections or cell smears. Recommended for H398 is the alkaline phosphatase-anti-alkaline-phosphatase (APAAP)or peroxidase anti-peroxidase (PAP) procedures or the three stage immunoperoxidase technique on acetone-fixed cryostat sections. H398 may be used at a concentration of 1-0.5mg/ml for cryostat sections)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Species Reactivity Human | Specificity Recognizes membrane-bound and soluble TNF-R1. | UniProt ID P19438 | Unit of Measure (UM) µg
