Product Name :
Anti-CD20, Human antibody
Applications:
ELISA,Flow Cyt
Reactivity :
Human
Conjugate:
Unconjugated
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-CD20, Human antibody is designed for detecting human CD20 specifically. Based on ELISA and/or FCM, Anti-CD20, Human antibody reacts with human CD20 specifically. | Immunogen: Recombinant human CD20 | Host: Alpaca pacous | Isotype: Human IgG1 | Conjugate: Unconjugated | Specificity: Human CD20 | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C, (Avoid freeze / thaw cycles) | Background:CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation.{{1515888-53-5} medchemexpress|{1515888-53-5} Purity & Documentation|{1515888-53-5} In Vitro|{1515888-53-5} manufacturer} After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment.{{1643125-33-0} web|{1643125-33-0} Protocol|{1643125-33-0} In Vivo|{1643125-33-0} manufacturer} Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.PMID:30860698
Description2 :
Anti-CD20, Human antibody is designed for detecting human CD20 specifically. Based on ELISA and/or FCM, Anti-CD20, Human antibody reacts with human CD20 specifically.
Immunogen:
Recombinant human CD20
Host :
Alpaca pacous
Isotype:
Human IgG1
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C, (Avoid freeze / thaw cycles)
Function:
ELISA: 1:4,000-1:10000Flow Cytometry：1:200-1:1000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
