And 50uC; lower temperatures affected the reaction rate while higher temperatures

And 50uC; lower temperatures affected the reaction rate while higher temperatures increased the loss of the analytes via MTBE volatilization. Derivatization time had noeffect on the derivatization efficiency of BAA, and showed a negative effect on that of CAA; however, the interaction between time and temperature weakened this negative effect. Derivatization efficiency of DXAAs and TXAAs increased with increasing derivatization time. The results were consistent with a previous study, wherein higher derivatization efficiency of HAA9, especially TXAAs, was achieved at longer derivatization time and higher temperature [25]. The application of the Derringer function [34], based on constructing a desirability function (obtained by calculating the individual desirability (di) and overall desirability (D), which is the weighted geometric average of the di) for each individual response, indicated the presence of an optimal result, which corresponded to 160 min of derivatization time and 40uC temperature.Figure 6. Chromatogram of IAA and HAA9. The concentration of each HAA was 10 mg/L and that of IAA was 1.0 mg/L. Numerals 1 to 11 represent CAA, BAA, DCAA, the internal standard (1, 2-dibromopropane), IAA, TCAA, BCAA, DBAA, BDCAA, CDBAA and TBAA, respectively. doi:10.1371/journal.pone.0060858.gPLOS ONE | www.Bromhexine hydrochloride plosone.Anti-Mouse TCR gamma/delta Antibody orgDetecting IAA, IF, THM4, and HAA9 in WaterTable 4. Performance and validation of the LLE-GC-ECD method for IF, THM4 and IAA, HAA9poundLinearity (mg/L)R2aLOD (mg/L)LOQ (mg/L)Recovery( ,n = 6) LowbRepeatability (RSD, , n = 6)cHighIntra-dayd LowbInter-daye Highc 5.0 5.6 4.7 3.4 5.5 4.2 7.0 3.3 7.1 6.7 1.9 6.5 2.8 5.0 4.1 #15 Low 8.8 3.8 4.0 7.7 7.9 3.4 9.7 4.2 4.9 2.5 6.2 4.5 5.0 4.6 3.6 #bHighc 4.4 7.0 3.5 5.2 7.9 7.0 6.1 7.7 7.7 4.9 6.5 3.7 2.5 2.3 2.7 #IAA CAA BAA DCAA BCAA DBAA TCAA BDCAA CDBAA TBAA IF CF BDCM CDBM BF EPAfa 2 b0.0150.0 0.550.0 0.150.0 0.0150.0 0.0150.0 0.0250.0 0.0150.0 0.150.0 0.0250.0 0.550.0 0.020.0 0.0100.0 0.010.0 0.010.0 0.010.0 0.9990 0.9996 0.9999 0.9994 0.9993 0.9999 0.9998 0.9990 0.9990 0.9991 0.9994 0.9982 0.9996 0.9998 0.9995 0.004 0.2 0.01 0.006 0.004 0.004 0.006 0.04 0.01 0.1 0.005 0.005 0.001 0.0008 0.002 0.01 0.4 0.08 0.008 0.01 0.02 0.008 0.08 0.02 0.4 0.02 0.01 0.008 0.008 0.008 106.3 101.0 99.1 96.2 94.2 100.4 104.2 105.7 92.4 92.1 98.8 98.6 98.0 100.5 101.3 8086.6 96.5 99.2 98.8 98.1 100.0 96.3 97.PMID:24238102 8 99.9 92.8 95.4 100.6 103.3 102.7 100.1 805.3 8.9 7.6 4.5 8.9 8.0 4.5 5.6 6.7 6.4 2.9 5.0 6.2 5.7 5.5 #R The correlation coefficient of the standard calibration curve. Low means low level of compound. IAA is 0.01 mg/L and HAA9 is 1.0 mg/L, while IF is 0.04 mg/L and THM4 is 2.0 mg/L. c High means high level of compound. IAA is 0.1 mg/L and HAA9 is 8.0 mg/L, while IF is 0.5 mg/L and THM4 is 12.0 mg/L. d Intra-day RSD was determined by analyzing six replicates for each level on the same day. e Inter-day RSD was performed by analyzing six replicates for 6 days. f This line shows the quality control standards of U.S. EPA [278]. doi:10.1371/journal.pone.0060858.tThe Volume of Organic Solvent (MTBE)In order to investigate the effect of organic solvent volume on derivatization efficiency, a single factor experiment with threelevel comparative analysis was used with MTBE volumes of 2, 3 and 4 mL. The response variable was the recovery of each HAA compound. The P value of normality and homogeneity of variance test was greater than 0.05, which suggested the data could be analyzed by one-way ANOVA. The results.