D by tooth eruption, replacement, and wear. The approval in the
D by tooth eruption, replacement, and wear. The approval in the Ethics Committee/Welfare Authority was not needed, as all 3-Chloro-5-hydroxybenzoic acid site samples have been taken postmortem. 2.two. Approaches 2.2.1. Serological Procedures Two commercial test kits had been utilised for the serological detection of T. gondii through the 2-year period. Samples from 2016 were tested using the ID ScreenToxoplasmosis Indirect Multi-Species kit (IDVET, Montpellier, France). Samples from 2017 were tested applying pigtypeToxoplasma Ab (Qiagen, Hilden, Germany). Each ELISA kits are appropriate for the detection of antibodies from wild boar serum and assays have been performed in accordance with the manufacturer’s instructions. Doubtful outcomes were regarded as damaging. 2.2.2. Statistical Analyses Information had been summarized as frequencies . Differences among groups (constructive vs. adverse) for gender (male, female), age (four categories: 0 year, 1 years, two years, 3 years), weight (five categories: 00 kg, 200 kg, 400 kg, 600 kg, 80 kg) and area (12 regions) (Table 1) have been tested applying a chi-squared test with Yates continuity correction. The multivariate analysis was performed utilizing a binary logistic regression. Random intercept by area was incorporated within the model to account for the prospective effect of theAnimals 2021, 11,3 ofregion (as a consequence of the massive quantity of regions, area was not viewed as as a fixed impact). Because of high collinearity among age and weight, three multivariate models had been fitted: (1) model such as gender, age, and weight as fixed
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