Step 17 above with a second cuff. Advance the cuff to a point just beyond

Step 17 above with a second cuff. Advance the cuff to a point just beyond the get started on the tapered section with roughly 1? mm in between the very first and second cuffs (Figure 3). Place the catheter around the catheter gauge card lining up the two cuffs with all the marks labeled cuff 1 and cuff 2. Making use of the razor blade cut the distal, compact end of tapered section of your tubing square across the bottom at the line marked “tip”. Cut the proximal, full diameter end of the catheter at the line marked “button” (A catheter gauge card and completed catheter with catheter connection post attached because it could be when implanted inside a rat are shown in Figure 4). (Optional) Location a modest volume of cyanoacrylate glue on the manage finish of your wooden swab and dab a small amount of glue on every cuff exactly where it meets the catheter tubing making certain to use the minimal quantity of glue feasible. When dried the glue really should be virtually undetectable This step just isn’t expected nor advisable if both cuffs are tight on the catheter tubing and usually do not slide unless considerable force is applied.Sterilize catheters in accordance with specifications of one’s institution’s Institutional Animal Care and Use Committee (IACUC). Polyurethane material is just not compatible with steam or dry heat sterilization. Ideal final results are obtained by ethylene oxide sterilization of catheters placed in sterilization pouch peel packs.Assistance PROTOCOL 5: SELF-ADMINISTRATION CATHETER IMPLANTATION Within the RATA previous protocol described a technique for implantation of a PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21114769 chronic indwelling jugular catheter in rats ((±)-BMS6462 price Thomsen and Cane, 2005). That protocol is suitable for the implantation of jugular catheters necessary for reinstatement research. Resulting from deviations in catheter building and implantation techniques, an alternative catheter implantation protocol optimized for polyurethane catheters and commercially-obtained catheter connection pedestals is provided. It is actually worth noting that modest deviations in surgical tactics, ordering of protocol methods and use of alterative instruments greater suited to a person surgeon are usually not problematic provided an acceptable outcome is achieved.Temperature regulated animal heating pad (e.g. Gaymar Mul ad? Deltaphase?Isothermal Pad or equivalent)Curr Protoc Neurosci. Author manuscript; obtainable in PMC 2013 October 01.Beardsley and SheltonPageSterilized jugular catheter (see Support Protocol four) Two 1 ml tuberculin syringes with needles 500 ml bag of 0.9 sterile saline Sterile surgical gloves Steam sterilized double wrapped surgical pack consisting of: 1 each ?straight mosquito hemostats 1 each ?curved mosquito hemostatswatermark-text watermark-text watermark-text1 each and every ?1×2 teeth little Adson forceps 1 each and every ?fine carbide blade straight scissors 1 every ?fine Dumont forceps 1 every ?fine 1×2 teeth straight Graefe or iris forceps four every – 1×1 gauze pads four each and every – wooden manage swabs 1 every single – 12×12 inch paper surgical drape 1 every ?#2 tapered Kalt suture needle two each ?10 cm length of four? braided silk or nylon suture material 4 each and every ?4×4 gauze pads (for manipulating non-sterile objects) eight each – 7.five mm Michel suture clips (or alternate closure materials) 1 each and every ?five cm length of 0.040 inch ID Tygon?tubing 1 each ?blunt 25 gauge needle 1 each ?catheter introducer (see Help Protocol 2) 1 each ?catheter connection pedestal (#313-000BM, PlasticsOne) Surgical procedure 1. Anesthetize the rat and prepare surgical websites (see Thomsen and Cane, 2005). Decision of an acceptable anesthesia.