product name Simvastatin
Description: Simvastatin (also known as MK-0733 and SIM) is a lactone prodrug and competitive inhibitor of HMG-CoA reductase with Ki of 0.1-0.2 nM in cell-free assays. It is used for treating coronary heart disease, hyperlipidemia, hypercholesterolemia, atherosclerosis and stroke. Simvastatin is biologically inactive, it has to be hydrolyzed it into the β-hydroxyacid form which is an inhibitor of 3-hydroxy-3-methyl glutaryl coenzyme A (HMG CoA) reductase.
References: Drugs. 1988;36 Suppl 3:72-82; Jpn J Pharmacol. 1989 Jan;49(1):125-33; Biochim Biophys Acta. 1990 Feb 23;1042(3):365-73.
418.57
Formula
C25H38O5
CAS No.
79902-63-9
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 83 mg/mL (198.3 mM)
Water: <1 mg/mL
Ethanol: 83 mg/mL (198.3 mM)
Solubility (In vivo)
2% DMSO+30% PEG 300+5% Tween80+ddH2O: 10 mg/mL
Synonyms
MK-0733
other peoduct :
| In Vitro |
In vitro activity: Prior to use in cell assays, Simvastatin needs to be activated by NaOH in EtOH treatment. Simvastatin inhibits cholesterol synthesis in mouse L-M cell (fibroblast), rat H4II E cell (liver), and human Hep G2 cell (liver) with IC50 of 19.3 nM, 13.3 nM and 15.6 nM, respectively. Simvastatin treatment leads to a dose-dependent increase in serine 473 phosphorylation of Akt within 30 minutes, with maximal phosphorylation occurring at 1.0 µM. Simvastatin (1.0 μM) enhances phosphorylation of the endogenous Akt substrate endothelial nitric oxide synthase (eNOS), inhibits serum-free media undergo apoptosis and accelerates vascular structure formation. Simvastatin displays anti-inflammatory effects in vitro. Simvastatin (10 μM) reduces anti-CD3/anti-CD28 antibody-stimulated proliferation of PB-derived mononuclear cells and synovial fluid cells from rheumatoid arthritis blood, as well as IFN-γ release. Simvastatin (10 μM) suppresses cell-mediated macrophage TNF-γ release induced via cognate interactions by ~30%. Kinase Assay: Cell Assay: In mouse L-M cells (fibroblast), rat H4IIE cells (liver) and human Hep G2 cells (liver), Simvastatin inhibited cholesterol synthesis with the IC50 values of 19.3 nM, 13.3 nM and 15.6 nM, respectively. |
|---|---|
| In Vivo | Simvastatin orally administration inhibits the conversion of radiolabeled acetate to cholesterol with IC50 of 0.2 mg/kg. Simvastatin (4 mg/day) orally administration for 13 weeks to rabbits fed an atherogenci cholesterol-rich diet, returns the cholesterol-induced increases in total cholesterol, LDL-cholesterol and HDL-cholesterol to normal level. Simvastatin (6 mg/kg) produces an increase in LDL receptor-dependent binding and increases the number of hepatic LDL receptors in rabbits fed a diet containing 0.25% cholesterol. Simvastatin influences inflammation independent of its effect on plasma cholesterol level. In cynomolgus monkeys consumed an atherogenic diet, Simvastatin (20 mg/kg/day) induces a 1.3-fold less macrophage content in lesions, and 2-fold less vascular cell adhesion molecule-1, interleukin-1beta, and tissue factor expression, companied by a 2.1-fold increases in lesional smooth muscle cell and collagen content. |
| Animal model | Dogs |
| Formulation & Dosage | 50 mg/kg/d; p.o. |
| References | Drugs. 1988;36 Suppl 3:72-82; Jpn J Pharmacol. 1989 Jan;49(1):125-33; Biochim Biophys Acta. 1990 Feb 23;1042(3):365-73. |
