product name Sal003
Description: Sal003 is a potent and cell-permeable eIF-2α phosphatase inhibitor. Sal003 increases eIF2α phosphorylation status by blocking eIF2a phosphatases in cells. In mouse embryonic fibroblasts (MEFs), Sal003 causes dissociation of polysomes by increasing eIF2a phosphorylation. Sal003 impairs late-long-term potentiation (L-LTP) in an ATF4-dependent mode in hippocampal slices from WT mice. In addition, eIF2α phosphorylation by Sal003 also enhances apoptotic signaling induced by subtilase cytotoxin (SubAB).
References: Mol Biol Cell. 2006 Nov;17(11):4632-44; Cell. 2007 Apr 6;129(1):195-206.
463.21
Formula
C18H15Cl4N3OS
CAS No.
1164470-53-4
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 93 mg/mL (200.78mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
Solubility (In vivo)
Synonyms
other peoduct :
| In Vitro |
In vitro activity: Sal003 increases eIF2α phosphorylation status by blocking eIF2a phosphatases in cells. In mouse embryonic fibroblasts (MEFs), Sal003 causes dissociation of polysomes by increasing eIF2a phosphorylation. Sal003 impairs late-long-term potentiation (L-LTP) in an ATF4-dependent mode in hippocampal slices from WT mice. In addition, eIF2α phosphorylation by Sal003 also enhances apoptotic signaling induced by subtilase cytotoxin (SubAB). Kinase Assay: Cell Assay: Sal003 specifically prevents dephosphorylation of eIF2α by blocking eIF2α phosphatases. In Sal003-treated cells polysomes dissociated and consistent with this β-actin mRNA shifted to lighter fractions, reflecting the inhibition of general translation |
|---|---|
| In Vivo | Sal003 impairs spatial long-term memory formation in rats. |
| Animal model | Rats |
| Formulation & Dosage | Dissolved in DMSO and further diluted in 0.9% NaCl to a final DMSO concentration of 0.1%; 2 μL of 20 μM; Intrahippocampal Injection. |
| References | Mol Biol Cell. 2006 Nov;17(11):4632-44; Cell. 2007 Apr 6;129(1):195-206. |
