product name Pyridostatin Trifluoroacetate
Description: Pyridostatin Trifluoroacetate is a G-quadruplexe stabilizer with Kd of 490 nM in a cell-free assay, which targets a series of proto-oncogenes including c-kit, K-ras and Bcl-2. Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC.
References: Nat Chem Biol. 2012 Feb 5;8(3):301-10; Nat Chem Biol. 2014 May;10(5):358-64.
596.64 (free base basis)
Formula
C31H32N8O5·xC2HF3O2
CAS No.
1085412-37-8 (free base)
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 100 mg/mL
Water: 100 mg/mL
Ethanol: 30 mg/mL
Solubility (In vivo)
Synonyms
other peoduct :References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19411764
| In Vitro |
In vitro activity: Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC. Pyridostatin decreases EBNA1 synthesis via inhibition of G-quadruplexes. Kinase Assay: Cell Assay: Cells (MRC-5–SV40 cells and various cancer cell lines) are plated at equal confluence and are left either untreated or were treated with 2 μM pyridostatin continually during the indicated time before harvesting the cells. Cells from individual plates are trypsinized and counted in a Coulter counter. Graphs represent the total cell numbers at each time interval, and the error bars represent the s.e.m. Data represent three independent experiments. |
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| In Vivo | |
| Animal model | |
| Formulation & Dosage | |
| References | Nat Chem Biol. 2012 Feb 5;8(3):301-10; Nat Chem Biol. 2014 May;10(5):358-64. |
