product name OAC1
Description: OAC-1 (also known as BAS 00287861) is an Oct-4 activator which enhances and accelerates iPSC reprogramming in the presence of 4F (Oct-4, Sox-2, c-Myc and GKLF) by ~20-fold (relative to 4F by itself). OAC-1 upregulates mRNA expression of Oct-4, Sox-2 and Nanog, as well as Tet1. It has shown no effects, however, on the p53-p21 pathway or Wnt-β-catenin signaling. In luciferase reporter assays, OAC1 activated both human Oct4 and Nanog promoter-driven luciferase reporter genes.
References: Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20853-8.
237.26
Formula
C14H11N3O
CAS No.
300586-90-7
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 47 mg/mL (198.1 mM)
Water: <1 mg/mL
Ethanol: 21 mg/mL (88.5 mM)
Solubility (In vivo)
Synonyms
BAS 00287861
other peoduct :
| In Vitro |
In vitro activity: OAC1 at 1 μM enhances reprogramming efficiency by activating both Oct4 and Nanog promoter-driven luciferase reporter genes. Furthermore, OAC1 enhances the pluripotent stem cells (iPSC) reprogramming efficiency and accelerates the reprogramming process in the quartet reprogramming factors (Oct4, Sox2, c-Myc, and Klf4) treated mouse embryonic fibroblasts (MEFs). The iPSC colonies derived using OAC1 along with the quartet factors exhibits typical ESC morphology, gene-expression pattern, and developmental potential. OAC1 seems to enhance reprogramming efficiency via increasing transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. While it doesn’t inhibit the p53-p21 pathway or activate the Wnt-β-catenin signaling. OAC1 may be used to enhance the reprogramming of somatic cells to a pluripotent state. Kinase Assay: Cell Assay: The Oct4-luc or Nanog-luc cells are treated with compound OAC1 at 1 μM concentration. Luciferase reporter assays are performed 24 h after OAC1 treatment. |
|---|---|
| In Vivo | In mouse embryonic fibroblasts (MEFs) with the 4F reprogramming quartet Oct4, Klf4, Sox2, and c-Myc, OAC1 enhanced reprogramming efficiency and increased the number of colonies with ESC-like morphology by twofold or more. Also, OAC1 increased induced pluripotent stem cells (iPSC)-like colonies. OAC1 had no effects on the Wnt signaling and p53-p21 expression. In MEFs, OAC1 activated the expression of endogenous Oct4 and Nanog. Also, OAC1 increased the expression of Sox2 and Tet1. |
| Animal model | |
| Formulation & Dosage | |
| References | Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):20853-8. |
