product name N6022
Description: N6022 is a selective, first-in-class and reversible inhibitor of S-nitrosoglutathione reductase (GSNOR) with IC50 of 8 nM and Ki of 2.5 nM. Inhibition of GSNOR causes the accumulation of GSNO which acts as a vasodilator and anti-inflammatory factor. N6022 presents an IC50 value of 8nM in the GSNO reduction assay and 32nM in the HMGSH oxidation assay. The Ki values are 2.5nM and 3.1nM, respectively. N6022 is selective against GSNOR over other human ADH enzymes. The IC50 values are 21μM, 67μM and 0.5μM for ADH IB, ADH II and ADH IV, respectively.
References: Biochemistry. 2012 Mar 13;51(10):2157-68; ACS Med Chem Lett. 2011 Mar 11;2(5):402-6.
414.46
Formula
C24H22N4O3
CAS No.
1208315-24-5
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 82 mg/mL (197.8 mM)
Water: <1 mg/mL
Ethanol: 5 mg/mL (12.1 mM)
Solubility (In vivo)
Synonyms
other peoduct :
| In Vitro |
In vitro activity: N6022 shows limited off-target activity and low cytotoxicity toward A549 epithelial lung cells. Kinase Assay: N6022 is a first-in-class inhibitor of GSNOR. Inhibition of GSNOR causes the accumulation of GSNO which acts as a vasodilator and anti-inflammatory factor. N6022 presents an IC50 value of 8nM in the GSNO reduction assay and 32nM in the HMGSH oxidation assay. The Ki values are 2.5nM and 3.1nM, respectively. N6022 is selective against GSNOR over other human ADH enzymes. The IC50 values are 21μM, 67μM and 0.5μM for ADH IB, ADH II and ADH IV, respectively. N6022 also shows no effect on the NADPH-dependent enzyme, human carbonyl reductase, with IC50 value of 221μM. Currently, N6022 is under clinical studies for the treatment of inflammatory lung diseases. Cell Assay: |
|---|---|
| In Vivo | N6022 (30 mg/kg iv or po) reduces bronchoconstriction and pulmonary inflammation in a mouse model of asthma. |
| Animal model | Mouse model of asthm. |
| Formulation & Dosage | Dissolved in 5% 2-hydroxypropyl-beta cyclodextrin in PBS (i.v.); and 1% carboxymethyl cellulose (p.o.) |
| References | Biochemistry. 2012 Mar 13;51(10):2157-68; ACS Med Chem Lett. 2011 Mar 11;2(5):402-6. |
