product name Isoxazole 9 (ISX-9)
Description: Isoxazole 9 (also known as Isx-9) is a synthetic promotor of adult neurogenesis by triggering neuronal differentiation of adult neural stem/precursor cells (NSPCs). Isx-9 promotes neurogenesis in vivo, enhancing the proliferation and differentiation of hippocampal subgranular zone (SGZ) neuroblasts, and the dendritic arborization of adult-generated dentate gyrus neurons. Isx-9 also improves hippocampal function, enhancing memory in the Morris water maze. Notably, Isx-9 enhances neurogenesis and memory without detectable increases in cellular or animal activity or vascularization.
References: PLoS One. 2015 Sep 25;10(9):e0138724; FASEB J. 2012 Aug;26(8):3148-62.
234.27
Formula
C11H10N2O2S
CAS No.
832115-62-5
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 46 mg/mL (196.4 mM)
Water: <1 mg/mL
Ethanol: 13 mg/mL (55.5 mM)
Solubility (In vivo)
5% DMSO+55% PEG 300+ddH2O: 10mg/mL
Synonyms
other peoduct :
| In Vitro |
In vitro activity: In NSPCs, isoxazole 9 increases cell number, and promotes cell differentiation. In OPCs, isoxazole 9 induces cell damage. In outgrowth EPCs, isoxazole 9 decreases tube formation without effect on early EPCs. Kinase Assay: Cell Assay: Cells are treated with different concentrations of Isx-9 (0, 6.25, 12.5, 25, or 50 μM), and 2 days later, cell numbers are counted in a blinded manner to evaluate the effects of Isx-9 on the proliferation/survival of four types of progenitor/precursor cells. |
|---|---|
| In Vivo | In mice, isoxazole 9 (20 mg/kg, i.p.) crosses the BBB and increases proliferation of neuroblasts and neurogenesis via Mef2-specific mechanisms in the hippocampal SGZ. Isoxazole 9 (20 mg/kg, i.p.) also increases differentiation and dendritic complexity of immature neurons and improves memory. In MWM, isoxazole 9 improves spatial memory. |
| Animal model | Transgenic Nkx2.5-luciferase reporter mice |
| Formulation & Dosage | Formulated in 30 % (2-hydroxypropyl)-β-cyclodextrin; 20 mg/kg; i.p. injection |
| References | PLoS One. 2015 Sep 25;10(9):e0138724; FASEB J. 2012 Aug;26(8):3148-62. |
