product name ID-8
Description: ID-8 is a small molecule DYRK inhibitor that sustains embryonic stem cell self-renewal in long-term culture. In HES2 cells, ID-8 (0.5 μM) increased hESC survival by 1.1%. The combination of ID-8 and Wnt3a increased survival by 1.7% and completely inhibited Wnt-induced morphological differentiation. Also, ID-8 significantly reduced the expression of differentiation marker gene GATA6, GSC, SOX17 and CDX2 induced by Wnt. In hESCs, ID-8 increased Wnt-mediated hESC proliferation and survival via inhibition of DYRKs. Also, DYRK family is direct targets of ID-8.
References: Biosci Biotechnol Biochem. 2008 May;72(5):1242-8; Stem Cells Transl Med. 2012 Jan;1(1):18-28.
298.29
Formula
C16H14N2O4
CAS No.
147591-46-6
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 60 mg/mL (201.1 mM)
Water: <1 mg/mL
Ethanol: 2 mg/mL (6.7 mM)
Solubility (In vivo)
Synonyms
other peoduct :
| In Vitro |
In vitro activity: ID-8 reversibly maintains the long-term culture of ESCs by sustaining Nanog gene expression through Sox2-Oct3/4 activation. ID-8 enhances Wnt-mediated hESC survival and proliferation via inhibition of DYRKs. The mechanism study indicates that ID-8 maintains the undifferentiated state in the presence of Wnt by modulating Wnt/β-catenin signaling and enhancing the CBP/β-catenin association in hESCs. Kinase Assay: Cell Assay: Briefly, the hESCs in feeder-free culture are dissociated completely with 0.05% trypsin-EDTA and seeded at 104 cells per well in Matrigel-coated 6-well culture plates and cultured in MEF-CM. Various concentrations of Wnt3, IQ-1, ID-8, and/or ICG-001 are supplemented into the culture media at the onset of seeding and then continuously until the end of culturing. For all assays, the cell and colony morphology are examined under a microscope, and the replating efficiency is examined by counting the number of colonies after 7 days of culture. |
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| In Vivo | |
| Animal model | |
| Formulation & Dosage | |
| References | Biosci Biotechnol Biochem. 2008 May;72(5):1242-8; Stem Cells Transl Med. 2012 Jan;1(1):18-28. |
