product name Hydroxyurea
Description: Hydroxyurea (also known as NCI-c04831, NSC32065) is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of hydroxyurea for laboratory strains of HIV-1 in activated PBMC is 0.4 mM.
References: Clin Infect Dis. 2000 Jun;30 Suppl 2:S193-7; Clin Infect Dis. 2000 Jun;30 Suppl 2:S143-50.
76.05
Formula
CH4N2O2
CAS No.
127-07-1
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 15 mg/mL (197.2 mM)
Water: 15 mg/mL (197.2 mM)
Ethanol: <1 mg/mL
Solubility (In vivo)
Synonyms
NCI-c04831, NSC32065
other peoduct :References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19413330
| In Vitro |
In vitro activity: Hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of hydroxyurea for laboratory strains of HIV-1 in activated PBMC is 0.4 mM. Hydroxyurea was also found to be synergistic with the nucleoside reverse transcriptase inhibitor didanosine and to inhibit HIV-1 replication in activated PBMC; this inhibition may be due to a reduction in deoxynucleoside triphosphate pool sizes. Hydroxyurea has been shown to sensitize didanosine-resistant mutants. Hydroxyurea has demonstrated activity in the treatment of sickle cell anemia by increasing the production of fetal hemoglobin, which reduces hemolysis in patients with this disease. Hydroxyurea exerts its cytostatic effect through inhibition of ribonucleotide reductase—the rate-limiting enzyme responsible for the conversion of ribonucleotides to deoxyribonucleotides, which are essential for DNA synthesis. As a result, cellular division is arrested in the S phase Kinase Assay: Cell Assay: Erythroid cells obtained from peripheral blood of the same patients(Thirteen β-Thal/HbE patients are treated with hydroxyurea orally for 2 years at a starting dose of 5 mg/kg/day for 5 days/week with escalation to a maximum of 10 mg/kg/day) 1 year after they had stopped hydroxyurea treatment are treated with hydroxyurea in vitro.Treatment of cells performs in primary culture with 30 μM hydroxyurea for 96 hours. |
|---|---|
| In Vivo | |
| Animal model | Female athymic (nu/nu) nude mice(used for xenograft model) |
| Formulation & Dosage | Dissolved in saline; 1500 mg/kg; i.p. injection |
| References | Clin Infect Dis. 2000 Jun;30 Suppl 2:S193-7; Clin Infect Dis. 2000 Jun;30 Suppl 2:S143-50. |
