product name Diosmetin
Description: Diosmetin (also known as DIO) is a natural flavonoid found in spearmint, oregano, and many other plants. Diosmetin is an agonist of the aryl hydrocarbon receptor (AHR). It potently inhibited the enzyme activity of cytochrome P450 1A1 (CYP1A1) in a dose-dependent manner with an IC50 value of approximately 30 nM, in microsomes from MCF-7 cells.
References: Pharmacol Res. 1992 Dec;26(4):395-402; Cancer Res. 1998 Jul 1;58(13):2754-60.
300.26
Formula
C16H12O6
CAS No.
520-34-3
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 60 mg/mL (199.8 mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
Solubility (In vivo)
Synonyms
Luteolin 4-methyl ether
other peoduct :
| In Vitro |
In vitro activity: Diosmetin inhibits 3H-dopamine uptake in neuroblastoma cells, as well as in small-cell lung carcinoma cells, which is be responsible for the increased vascular tone observed in vivo after treatment with Diosmetin. Moreover, Diosmetin also removes iron from iron-loaded hepatocytes revealed a good relationship between this iron-chelating ability and the cytoprotective effect. Other studies proves that Diosmetin is a cytochrome P450 inhibitor, thus inhibiting carcinogen activation and and causing pharmacokinetic interactions with co-administered drugs metabolized. Kinase Assay: Cell Assay: In MCF-7 cells, at 24 h after the incubation of diosmetin, CYP1A1 mRNA was increased in a dose-dependent manner. In MCF-7 cells, diosmetin at 2.5 µM modestly increased CYP1A1 enzyme activity, with an activity increase in cells, while diosmetin at 5 µM did not increase the enzyme activity compared to controls in cells. Compared with controls, diosmetin dose-dependently increased the capacity of nuclear extracts to bind an oligonucleotide containing the AhR-binding sequence of CYP1A1. |
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| In Vivo | |
| Animal model | |
| Formulation & Dosage | |
| References | Pharmacol Res. 1992 Dec;26(4):395-402; Cancer Res. 1998 Jul 1;58(13):2754-60. |
