product name DMOG
Description: DMOG, also known as Dimethyloxaloylglycine, is a cell permeable antagonist of α-ketoglutarate cofactor and competitive inhibitor for HIF prolyl hydroxylase. It acts to stabilize HIF-1α expression at normal oxygen tensions in cultured cells, at concentrations between 0.1 and 1 mM. DMOG provides neuroprotection after traumatic brain injury. DMOG provides enhanced efficacy with reduced systemic exposure in a murine model of colitis.
References: Biochem J. 1994 Jun 1;300 ( Pt 2):525-30; J Physiol. 2004 Oct 1;560(Pt 1):21-6.
175.14
Formula
C6H9NO5
CAS No.
89464-63-1
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 35 mg/mL (199.8 mM)
Water: 35 mg/mL (199.8 mM)
Ethanol: 35 mg/mL (199.8 mM)
Solubility (In vivo)
Synonyms
Dimethyloxalylglycine
other peoduct :
| In Vitro |
In vitro activity: DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. DMOG reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC. Kinase Assay: Cell Assay: To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells. |
|---|---|
| In Vivo | DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. Up-regulation of hypoxia-inducible factor-1α by DMOG enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats. |
| Animal model | C57Bl6 Mice |
| Formulation & Dosage | 8 mg DMOG dissolved in 0.5 ml saline; 8 mg/mouse; i.p. injection |
| References | Biochem J. 1994 Jun 1;300 ( Pt 2):525-30; J Physiol. 2004 Oct 1;560(Pt 1):21-6; Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):112-8. |
