product name D-Luciferin
Description: D-Luciferin is a popular, cell-permeable bioluminescent substrate of luciferase in the presence of ATP with a Km of approximately 2 μM, it is used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications. D-luciferin could emit lights upon oxidative decarboxylation in the presence of ATP. D-luciferin provides a bioluminescent signal for in vivo and in vitro detection of cellular ATP levels. D-Luciferin chould be used to assay the expression of the luciferase gene linked to a promoter of interest. Alternatively, D-luciferin and luciferase can be used to assess ATP availability in cellular or biochemical assays. D-luciferin could be administrated intravenously or intraperitonealy.
References: J Clin Chem Clin Biochem. 1987 Aug;25(8):511-4; J Immunother Cancer. 2015 May 19;3:16.
280.32
Formula
C11H8N2O3S2
CAS No.
2591-17-5
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 56 mg/mL (199.8 mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
Solubility (In vivo)
Synonyms
other peoduct :
| In Vitro |
In vitro activity: D-luciferin reacts with luciferase, ATP and oxygen with light emission, and the light is detected by a sensitive photographic film, thereby permitting the visualization of the alkaline phosphatase-conjugated antibodies. Kinase Assay: Cell Assay: |
|---|---|
| In Vivo | In an immunocompetent mouse model of ovarian cancer, use of D-luciferin substrate and firefly luciferase preserves tumor-host immune interactions, since bioluminescent imaging is a more sensitive indication of tumor growth than weight gain. |
| Animal model | |
| Formulation & Dosage | |
| References | J Clin Chem Clin Biochem. 1987 Aug;25(8):511-4; J Immunother Cancer. 2015 May 19;3:16. |
