product name Blasticidin S HCl
Description: Blasticidin S HCl is a nucleoside antibiotic isolated from Stretomyces girseochromogenes, and acts as a DNA and protein synthesis inhibitor, it is used to select transfected cells carrying bsr or BSD resistance genes. In mouse leukemia L5178Y cells, blasticidin S inhibited protein synthesis and caused the degradation of presumptive mRNA [1]. Also, blasticidin S inhibited ribonuclease P activity from Dictyostelium discoideum and inhibited tRNA maturation in a dose dependent way.
References: J Biochem. 1966 Dec;60(6):632-42; Biochim Biophys Acta. 1977 Mar 2;475(1):14-22.
458.9
Formula
C17H27ClN8O5
CAS No.
3513-03-9
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: <1 mg/mL
Water: 91 mg/mL (198.3 mM)
Ethanol: <1 mg/mL
Solubility (In vivo)
Synonyms
other peoduct :References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19411820
| In Vitro |
In vitro activity: Blasticidin S inhibits protein synthesis by acting on the step of peptide transfer from peptidyl-sRNA to incoming aminoacyl-sRNA. Blasticidin S also significantly inhibits DNA synthesis independent of their effect on protein synthesis. Blasticidin S kills the cells more rapidly than G418, which makes blasticidin S-resistance gene (bsr) a selectable marker for mammalian cells. Besides, Blasticidin S also efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. Kinase Assay: Cell Assay: |
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| In Vivo | |
| Animal model | |
| Formulation & Dosage | |
| References | J Biochem. 1966 Dec;60(6):632-42; Biochim Biophys Acta. 1977 Mar 2;475(1):14-22. |
