product name Biochanin A
Description: Biochanin A (also known as 4-Methylgenistein), an natural isoflavone from Trifolium pratense, inhibits protein tyrosine kinase (PTK) of epidermal growth factor receptor with IC50 values of 91.5 μM. Biochanin A has been reported to inhibit protein tyrosine kinase (PTK) of epidermal growth factor receptor with IC50 values of 91.5 μM. Biochanin A inhibits the hydrolysis of 0.5 μM anandamide by mouse, rat and human fatty acid amide hydrolase (FAAH) with IC50 values of 1.8, 1.4 and 2.4 μM respectively.
References: J Biol Chem. 1987 Apr 25;262(12):5592-5; Br J Pharmacol. 2010 Jun;160(3):549-60.
284.26
Formula
C16H12O5
CAS No.
491-80-5
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 57 mg/mL (200.5 mM)
Water: <1 mg/mL
Ethanol: 9 mg/mL (31.7 mM)
Solubility (In vivo)
Synonyms
other peoduct :References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19420020
| In Vitro |
In vitro activity: Biochanin A has been reported to inhibit protein tyrosine kinase (PTK) of epidermal growth factor receptor with IC50 values of 91.5 μM. Biochanin A inhibits the hydrolysis of 0.5 μM anandamide by mouse, rat and human fatty acid amide hydrolase (FAAH) with IC50 values of 1.8, 1.4 and 2.4 μM respectively. Biochanin A inhibits both serum and EGF-stimulated growth of LNCaP and DU-145 cells (IC50 values from 8.0 to 27 micrograms/ml for serum and 4.3 to 15 micrograms/ml for EGF), but has no significant effect of the EGF receptor tyrosine autophosphorylation. Kinase Assay: Cell Assay: |
|---|---|
| In Vivo | In anaesthetized mice, both URB597 and biochanin A inhibited the spinal phosphorylation of extracellular signal-regulated kinase caused by the intraplantar injection of formalin. The effects of both URB597 and biochanin A were found to be significantly reduced by the CB1 receptor antagonist/inverse agonist AM251. In addition, in the tetrad test, biochanin A did not increase brain AEA concentrations, but produced a modest potentiation of the effects of 10 mg·kg-1 i.v. AEA. |
| Animal model | |
| Formulation & Dosage | |
| References | J Biol Chem. 1987 Apr 25;262(12):5592-5; Br J Pharmacol. 2010 Jun;160(3):549-60. |
