product name Asaraldehyde
Description: Asaraldehyde (also known as Asaronaldehyde) is a natural COX-2 inhibitor which exhibits 17-fold selectivity over COX-1. Asaraldehyde shows 3.32% of prostaglandin H endoperoxide synthase-1 (COX-1) inhibitory activity and 52.69% prostaglandin H endoperoxide synthase-2 (COX-2) inhibitory activity, respectively at 100 mg/mL. Asaraldehyde is a natural COX-2 inhibitor, exhibiting 17-fold selectivity over COX-1.
References: Lett Appl Microbiol. 2007 Apr;44(4):387-92; Phytother Res. 2003 Sep;17(8):976-9.
196.2
Formula
C10H12O4
CAS No.
4460-86-0
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 39 mg/mL (198.8 mM)
Water: <1 mg/mL
Ethanol: 16 mg/mL (81.5 mM)
Solubility (In vivo)
Synonyms
Asaronaldehyde
other peoduct :References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19409076
| In Vitro |
In vitro activity: Asaraldehyde is found to be the most abundant constituent, but is totally absent in its cultured broth and its natural host, C. kanehirae wood. On feeding with the acid-digested sawdust of C. kanehirae wood or vanillin to the broth for culture, Asaraldehyde is produced in both cultured broths. Asaraldehyde shows 3.32% of prostaglandin H endoperoxide synthase-1 (COX-1) inhibitory activity and 52.69% prostaglandin H endoperoxide synthase-2 (COX-2) inhibitory activity, respectively at 100 mg/mL. Asaraldehyde shows selectivity towards COX-2 enzyme inhibition at 100 µg/mL. The COX-2/COX-1 ratio for Asaraldehyde is 17.68 at 100 µg/mL compared to solvent control. Asaraldehyde down-regulates C/EBPβ, C/EBPδ, and C/EBPα. Asaraldehyde suppresses expression of PPARγ1 and PPARγ2. Asaraldehyde also significantly suppresses the expression of acetyl-CoA carboxylase (ACC). Kinase Assay: Cell Assay: 3T3-L1 cells are seeded in 96-well plates at a concentration of 104 /well. Twenty-four hours after seeding, the cells are treated with 100 μg/mL of Asaraldehyde for 24 hours or for the whole 8-day differentiation period. Fully differentiated adipocytes are also treated with 100 μg/mL of Asaraldehyde for 24 hours-72 hours to test the cytotoxicity. At the end of treatment, cells are cultured with MTT at a final concentration of 0.5 mg/mL for another 4 hours. The purple MTT formazan is dissolved by DMSO and the absorbance at 570 nm is taken with a spectrophotometer. The absorbance is proportional to the viability of adipocytes. |
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| In Vivo | |
| Animal model | |
| Formulation & Dosage | |
| References | Lett Appl Microbiol. 2007 Apr;44(4):387-92; Phytother Res. 2003 Sep;17(8):976-9. |
