Ere provided. To stabilize the identity of the nomen, we designate ZMB 8255A (an adult female, SVL 54 mm) as the lectotype. Referred Specimens.–Republic of Equatorial Guinea, Bioko Island: Bioko Norte BKT140 web Province: CAS 207615?9 (N 3.7110?E 8.6666? 55 m), 18 October 1998; Bioko Sur Province: ArenaPLOS ONE | DOI:10.1371/journal.pone.0142823 December 16,12 /Six New Species of African Clawed Frog (Xenopus)Table 3. Summary of lateral line counts Species subgenus Silurana X. calcaratus X. epitropicalis X. mellotropicalis X. tropicalis subgenus Xenopus ameiti group X. allofraseri X. Lurbinectedin chemical information amieti X. andrei X. boumbaensis X. jir.2010.0097 eysoole X. fraseri X. kobeli X. longipes X. parafraseri X. ruwenzoriensis laevis group X. poweri muelleri group X. borealis X. muelleri X. fischbergi 13.5 (10?6); 45 13.6 (11?6); 17 13.5 (12?5); 4 15.0 (11?8); 44 14.4 (11?8); 17 14.8 (11?6); 4 25.2 (21?1); 45 28.1 (24?2); 17 25.3 (24?6); 4 26.8 (19?1); 45 28.6 (24?4); 17 28.0 (26?1); 4 24.8 (18?9); 31 26.0 (17?2); 17 22.7 (22?4); 3 13.7 (13?5); 9 13.2 (11?6); 9 21.7 (19?8); 9 24.4 (18?9); 9 21.8 (19?5); 6 12.7 (11?5); 18 13.2 (11?7); 12 10.0 (10?0); 1 12.7 (10?6); 31 12.5 (10?4); 13 13.0 (13?3); 1 10.7 (10?2); 9 11.4 (10?3); 11 10.7 (9?3); 33 11.8 (11?4); 4 12.6 (10?5); 18 10.5 (8?5); 20 13.0 (13?3); 1 12.8 (11?5); 31 10.6 (9?3); 13 14.0 (13?5); 2 16.2 (14?9); 12 8.8 (8?0); 4 10.6 (8?3); 32 11.3 (10?2); 4 15.5 (13?7); 18 18.6 (14?5); 25 16.0 (16?6); 1 17.2 (14?1); 31 19.8 (17?3); 13 21.0 (21?1); 2 19.3 (16?2); 12 13.3 (8?6); 11 15.9 (11?0); 35 16.5 (14?8); 4 20.3 (18?3); 18 20.9 (18?6); 25 16.0 (16?6); 1 21.5 (18?5); 31 20.4 (18?3); 13 23.0 (21?5); 2 15.3 (13?1); 4 17.1 (14?1); 11 18.2 (15?1); 35 18.5 (17?9); 4 17.0 (17?7); 2 19.1 (13?7); 11 17.0 (17?7); 1 20.6 (17?5); 31 15.5 (13?8); 4 24.0 (24?4); 1 15.3 (13?1); 4 17.0 (17?7); 1 16.1 (14?9); 19 19.3 (18?2); 3 10.4 (9?3); 17 12.0 (12?2); 1 11.0 (12?2); 4 10.3 (8?2); 15 10.3 (9?2); 17 11.0 (11?1); 1 11.3 (10?3); 4 10.7 (9?2); 15 16.4 (15?9); 17 21.0 (21?1); 1 17.25 (16?9); 4 17.9 (16?1); 15 18.4 (15?1); 17 23.0 (23?3); 1 19.0 (16?9); 4 19.9 (17?2); 15 17.4 (14?1); 17 19.0 (19?9); 1 14.3 (10?8); 4 18.3 (16?1); 15 ER OLL MLL LLL VLLEye ridges (ER), oral lateral lines (OLL), medial lateral lines (MLL), lateral lateral lines (LLL), and ventral lateral lines (VLL) of representative species. Numbers indicate the average, range in parentheses, and the number of individuals counted. Data are lacking for species not listed. doi:10.1371/journal.pone.0142823.tBlanca Road: CAS 207752?3 and 207755?8 (N 3.5201? E 8.5859? 65 m), 207759?4 (N 3.5276? E 8.5794? 30 m), 14 October 1998; coll. L. G. Henwood, J. V. Vindum. Republic of Cameroon: South West Region, Bakingili, lava flow: NMP6V 74630/1-4 (two subadults, probably j.jebo.2013.04.005 females, and two adult males), 25 November 2009, NMP6V 74746 (adult female), 11 December 2005, (N 4.0689? E 9.0684? 180 m), coll. V. Gvozd . Diagnosis.–Xenopus calcaratus is a tetraploid species with a burst-type call that exhibits all of the morphological features of subgenus Silurana described above. Most individuals are a medium to dark brown with a pale interocular bar (more distinct in the Bioko population), and a rostrum tending to darker brown coloration than the rest of the dorsum. Specimens from Bioko Island often exhibit small scattered and irregularly shaped dark brown spots on the dorsum and hindlimbs, which has not been recorded in Cameroonian specimens. Xenopus calcaratus differs from other species of Silurana in th.Ere provided. To stabilize the identity of the nomen, we designate ZMB 8255A (an adult female, SVL 54 mm) as the lectotype. Referred Specimens.–Republic of Equatorial Guinea, Bioko Island: Bioko Norte Province: CAS 207615?9 (N 3.7110?E 8.6666? 55 m), 18 October 1998; Bioko Sur Province: ArenaPLOS ONE | DOI:10.1371/journal.pone.0142823 December 16,12 /Six New Species of African Clawed Frog (Xenopus)Table 3. Summary of lateral line counts Species subgenus Silurana X. calcaratus X. epitropicalis X. mellotropicalis X. tropicalis subgenus Xenopus ameiti group X. allofraseri X. amieti X. andrei X. boumbaensis X. jir.2010.0097 eysoole X. fraseri X. kobeli X. longipes X. parafraseri X. ruwenzoriensis laevis group X. poweri muelleri group X. borealis X. muelleri X. fischbergi 13.5 (10?6); 45 13.6 (11?6); 17 13.5 (12?5); 4 15.0 (11?8); 44 14.4 (11?8); 17 14.8 (11?6); 4 25.2 (21?1); 45 28.1 (24?2); 17 25.3 (24?6); 4 26.8 (19?1); 45 28.6 (24?4); 17 28.0 (26?1); 4 24.8 (18?9); 31 26.0 (17?2); 17 22.7 (22?4); 3 13.7 (13?5); 9 13.2 (11?6); 9 21.7 (19?8); 9 24.4 (18?9); 9 21.8 (19?5); 6 12.7 (11?5); 18 13.2 (11?7); 12 10.0 (10?0); 1 12.7 (10?6); 31 12.5 (10?4); 13 13.0 (13?3); 1 10.7 (10?2); 9 11.4 (10?3); 11 10.7 (9?3); 33 11.8 (11?4); 4 12.6 (10?5); 18 10.5 (8?5); 20 13.0 (13?3); 1 12.8 (11?5); 31 10.6 (9?3); 13 14.0 (13?5); 2 16.2 (14?9); 12 8.8 (8?0); 4 10.6 (8?3); 32 11.3 (10?2); 4 15.5 (13?7); 18 18.6 (14?5); 25 16.0 (16?6); 1 17.2 (14?1); 31 19.8 (17?3); 13 21.0 (21?1); 2 19.3 (16?2); 12 13.3 (8?6); 11 15.9 (11?0); 35 16.5 (14?8); 4 20.3 (18?3); 18 20.9 (18?6); 25 16.0 (16?6); 1 21.5 (18?5); 31 20.4 (18?3); 13 23.0 (21?5); 2 15.3 (13?1); 4 17.1 (14?1); 11 18.2 (15?1); 35 18.5 (17?9); 4 17.0 (17?7); 2 19.1 (13?7); 11 17.0 (17?7); 1 20.6 (17?5); 31 15.5 (13?8); 4 24.0 (24?4); 1 15.3 (13?1); 4 17.0 (17?7); 1 16.1 (14?9); 19 19.3 (18?2); 3 10.4 (9?3); 17 12.0 (12?2); 1 11.0 (12?2); 4 10.3 (8?2); 15 10.3 (9?2); 17 11.0 (11?1); 1 11.3 (10?3); 4 10.7 (9?2); 15 16.4 (15?9); 17 21.0 (21?1); 1 17.25 (16?9); 4 17.9 (16?1); 15 18.4 (15?1); 17 23.0 (23?3); 1 19.0 (16?9); 4 19.9 (17?2); 15 17.4 (14?1); 17 19.0 (19?9); 1 14.3 (10?8); 4 18.3 (16?1); 15 ER OLL MLL LLL VLLEye ridges (ER), oral lateral lines (OLL), medial lateral lines (MLL), lateral lateral lines (LLL), and ventral lateral lines (VLL) of representative species. Numbers indicate the average, range in parentheses, and the number of individuals counted. Data are lacking for species not listed. doi:10.1371/journal.pone.0142823.tBlanca Road: CAS 207752?3 and 207755?8 (N 3.5201? E 8.5859? 65 m), 207759?4 (N 3.5276? E 8.5794? 30 m), 14 October 1998; coll. L. G. Henwood, J. V. Vindum. Republic of Cameroon: South West Region, Bakingili, lava flow: NMP6V 74630/1-4 (two subadults, probably j.jebo.2013.04.005 females, and two adult males), 25 November 2009, NMP6V 74746 (adult female), 11 December 2005, (N 4.0689? E 9.0684? 180 m), coll. V. Gvozd . Diagnosis.–Xenopus calcaratus is a tetraploid species with a burst-type call that exhibits all of the morphological features of subgenus Silurana described above. Most individuals are a medium to dark brown with a pale interocular bar (more distinct in the Bioko population), and a rostrum tending to darker brown coloration than the rest of the dorsum. Specimens from Bioko Island often exhibit small scattered and irregularly shaped dark brown spots on the dorsum and hindlimbs, which has not been recorded in Cameroonian specimens. Xenopus calcaratus differs from other species of Silurana in th.
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