product name L-Mimosine
Description: L-Mimosine (also known as Leucenol) is a non-protein amino acid, and acts as an iron chelator. L-mimosine acted as an inhibitor for prolyl 4-hydroxylase in cultured vascular cells. L-mimosine dose-dependently inhibited the activity of prolyl hydroxylase in rat and human smooth muscle cells (SMC) and at concentrations of 400-500 µM decreased hydroxyprolyl generation by 80-90%. At that concentration, [3H]proline incorporation was decreased by < 20% in human SMC and was increased by 20% in rat SMC. These results indicated that L-mimosine quite specifically inhibited prolyl hydroxylation.
References: Am J Physiol Cell Physiol. 2012 Feb 15;302(4):C676-85; J Endod. 2012 Nov;38(11):1498-503; Am J Physiol Renal Physiol. 2007 Jan;292(1):F207-16.
198.18
Formula
C8H10N2O4
CAS No.
500-44-7
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: <1 mg/mL
Water: <1 mg/mL
10% NaHCO3 (aq): 38 mg/mL (191.74 mM)
Solubility (In vivo)
Synonyms
Leucenol
other peoduct :
| In Vitro |
In vitro activity: L-Mimosine arrests the cell cycle at the G1 phase in PC-3 cells and at the S phase in LNCaP cells, thus attenuating cell proliferation. L-Mimosine, as a prolyl hydroxylase (PHD) inhibitor, stimulates VEGF production, and increases their proangiogenic capacity in dental pulp-derived cells. Kinase Assay: In rats, it interfered with the reconstitution of the active human prolyl 4-hydroxylase with an IC50 value of 120 µM. Cell Assay: Cell proliferation in response to L-mimosine is measured using a [3H]thymidine incorporation assay. In this assay, 1 × 104 cells are cultured in each well of a 12-well plate in RPMI-1640 medium with 10% FCS and different concentrations (0–800 μM) of L-mimosine. After the required incubation periods (24 and 48 h), 0.5 μCi/ml of [3H]thymidine is added to each well of the 12-well plate. The cells are then incubated at 37°C in a humidified 5% CO2 atmosphere for 4 h. Cells are then washed twice with cold phosphate-buffered saline (PBS) and then with cold 5% trichloroacetic acid. Cells are lysed by adding 0.5 ml of 0.5 N NaOH. Then 400 μl of the solubilized cell solution are mixed with 4 ml of scintillation cocktail and counted in a liquid scintillation analyzer . Each sample was tested in quadruplicate. |
|---|---|
| In Vivo | L-mimosine (50 mg/kg i.p.) substantially upregulates HIF-1alpha expression in the kidneys, and remarkably enhances the natriuretic response to renal perfusion pressure in Sprague-Dawley rats. |
| Animal model | Sprague-Dawley rats |
| Formulation & Dosage | Dissolved in Na2CO3 solution, pH 8.5; 50 mg/kg; i.p. injection |
| References | Am J Physiol Cell Physiol. 2012 Feb 15;302(4):C676-85; J Endod. 2012 Nov;38(11):1498-503; Am J Physiol Renal Physiol. 2007 Jan;292(1):F207-16. |
