product name Optovin
Description: Optovin is a reversible photoactivated TRPA1 activator. It activates human TRPA1 via structure dependent photochemical reactions. In dorsal root ganglia (DRG) sensory neurons isolated from mice, optovin significantly activated 33% (35/105) of DRG neurons, which was not occured in the dark. In HEK293T cells transfected with the human TRPA1, optovin activated cells. Optovin activated TRPA1 channels through a photoactivated intermediate that reacted with redox-sensitive cysteine residues in the channel.
References: Nat Chem Biol. 2013 Apr;9(4):257-63.
315.41
Formula
C15H13N3OS2
CAS No.
348575-88-2
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 63 mg/mL (199.74 mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
Solubility (In vivo)
Synonyms
other peoduct :
| In Vitro |
In vitro activity: Optovin activates human TRPA1 via structure dependent photochemical reactions. Kinase Assay: Cell Assay: In HEK293T cells transfected with the human TRPA1, optovin activated cells. Optovin activated TRPA1 channels through a photoactivated intermediate that reacted with redox-sensitive cysteine residues in the channel. |
|---|---|
| In Vivo | Optovin enables real-time optical control of neurons, and also elicits nociceptive behaviors in adult mice. In animals with severed spinal cords, optovin treatment enables control of motor activity in the paralyzed extremities by localized illumination. |
| Animal model | Zebrafish and mice |
| Formulation & Dosage | Dissolved in DMSO; 50 μM (Zebrafish); 15 mM (mice); Treated on the ear (mice); Embryos are incubated in Optovin solution (Zebrafish) |
| References | Nat Chem Biol. 2013 Apr;9(4):257-63. |
