product name O4I1
Description: O4I1 is a potent inducer of Oct3/4 and can enhance Oct3/4 expression. Oct3/4 is specifically expressed in the ES cells, epiblast, preimplantation embryos, and germ cells, et al. O4I1 not only promoted expression and stabilization of Oct3/4 but also enhanced its transcriptional activity in diverse human somatic cells, implying the possible benefit from using this class of compounds in regenerative medicine.
References: J Med Chem. 2015 Jun 25;58(12):4976-83.
253.30
Formula
C16H15NO2
CAS No.
175135-47-4
Storage
-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)
DMSO: 51 mg/mL (201.3 mM)
Water: <1 mg/mL
Ethanol: <1 mg/mL
Solubility (In vivo)
Synonyms
other peoduct :
| In Vitro |
In vitro activity: O4I1 enhances Oct3/4 gene expression by approximately 2.5- and 4-fold at 10 μM and 20 μM, respectively, increases Oct3/4 protein levels, and promotes Oct3/4 mediated transcriptional activation. Kinase Assay: Cell Assay: When treated the synthesized cDNA from RNA isolated from the HEK293 cells with 10 µM and 20 µM O4I1 for 72 hrs, the Oct3/4 mRNA levels increased about 2.5- and 4-fold respectively. In human adult primary fibroblast (HF) and human neonatal foreskin fibroblast (HFF) cell lines, treatment with 10 µM O4I1 increased the gene and protein expression level of Oct3/4 after 72 hrs incubation in HF cells and 7 days in HFF cells. The derivatives of O4I1 such as compound 2, showed a time-dependent activation of Oct3/4 and markedly elevated the efficiency of Oct3/4 induction. |
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| In Vivo | |
| Animal model | |
| Formulation & Dosage | |
| References | J Med Chem. 2015 Jun 25;58(12):4976-83. |
