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product name Z-VAD-FMK


Description: Z-VAD-FMK, an inhibitor of ICE-like proteases, inhibits apoptosis in THP.1 cells induced by diverse stimuli and Fas antigen-induced apoptosis in Jurkat T-cells. It inhibits apoptosis by blocking the activation of proCPP32 into its active form, rather than by preventing the proteolytic action of CPP32 directly. Z- VAD-FMK inhibits the formation of large kilobasepair fragments of DNA induced by diverse stimuli. Z-VAD-FMK had little or no effect on STS-induced necrotic cell death suggesting that the ICE-like protease activity was not involved in necrosis.

Reference: Biochem J. 1996 Apr 1;315 ( Pt 1):21-4; Leukemia. 1997 Aug;11(8):1238-44.



Molecular Weight (MW)

467.49
Formula

C22H30FN3O7
CAS No.

187389-52-2
Storage

-20℃ for 3 years in powder form
-80℃ for 2 years in solvent
Solubility (In vitro)

DMSO: 93 mg/mL (198 mM)
Water: <1 mg/mL (slightly soluble or insoluble)
Ethanol: 24 mg/mL warming (51 mM)
Solubility (In vivo)

 

other peoduct :References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/19404868

Z-VAD-FMK (10 mM) inhibits apoptosis in THP.1 cells. Z-VAD-FMK (10 μM) inhibits activation of PARP protease activity in control THP.1 cell lysates.
In Vivo The right ear swelling degree, weight differences and thickness between two ears in the 1.25 mML Z-VAD-FMK group were significantly lower than those of the negative control (NC). The levels of INF-γ and IL-2 in the ear skin lesions, the mean intensity of BrdU in T lymphocytes, and the percent of activation markers-positive T lymphocytes were all lower than those of NC.
Animal model  C57BL mice
Formulation & Dosage 1.25 mMl; Ear provocation
Reference Zhonghua Yi Xue Za Zhi. 2012 Jul 24;92(28):1992-6.

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