There was no significant colocalization between MMP-9 and podocyte marker proteins

s, but the half-life of IL-6 mRNA in YB-1-depleted macrophages was similar to that of control macrophages. YB-1 depletion did not affect TNF-a mRNA expression or stability. Calculation of mRNA band intensities also indicated that in YB-1-depleted macrophages, the relative amount of IL-6 mRNA increased more than two fold compared to control cells; however, we found that the half-life of IL-6 mRNA or TNF-a mRNA was unaffected. These results suggest that YB-1 negatively regulates intracellular IL-6 mRNA expression levels, but it is not due to an increase in RNA turnover. YB-1 actively exports cytosolic IL-6 mRNA to the extracellular space to control intracellular IL-6 mRNA levels in macrophages The localization of YB-1 affects IL-6 production Because YB-1 serves as a regulator in both pro- and antiinflammatory responses and its cellular distribution was quite different between macrophages and dendritic cells, we investigated whether this differential localization of YB-1 affects its ability to induce cytokine production in response to LPS. YB-1 was depleted in macrophages or BMDCs using YB-1-specific RNA interference. Unexpectedly, depletion of YB-1 enhanced LPS-induced production of IL-6 but not TNF-a in macrophages. Likewise, CpG-DNA-stimulated macrophages expressing YB-1-specific RNAi exhibited increased production of IL6 but not TNF-a. In contrast to macrophages, LPSexposed dendritic cells expressing YB-1 RNAi reduced IL-6 Functional Role of YB-1 in Controlling Intracellular IL-6 mRNA Levels lular space. Macrophages expressing 1235481-90-9 either control or YB-1 RNAi were incubated in serum-free medium in the absence or presence of LPS for 24 h. Culture medium was collected from the macrophages and then assayed for IL-6 mRNA by RT-PCR. Surprisingly, we found that IL-6 mRNA was secreted to the extracellular space from macrophages; in contrast, we did not observe any secreted TNF-a mRNA. Furthermore, intracellular and extracellular IL-6 mRNA levels PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19689163 correlated with YB-1 expression levels in macrophages. YB-1 depletion increased the amount of intracellular IL-6 mRNA and decreased levels of extracellular IL-6 mRNA. TNF-a mRNA levels were unaffected, as shown previously. We quantified the IL-6 mRNA band intensities from RT-PCR and showed that IL-6 mRNA increased in the intracellular space by 51% in YB-1-depleted macrophages and extracellular IL-6 mRNA levels decreased by 58%. Because YB-1 binds to single-stranded cisplatin-modified Y box DNA sequences and degrades DNA by its exonuclease activity, PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/19692147 we investigated whether YB-1 could also degrade RNA. We purified YB-1 by immunoprecipitation and then incubated the protein with oligonucleotides or poly-A tail RNAs. An in vitro degradation assay showed that YB-1 degraded oligonucleotides but not RNA, indicating that YB-1 does not have an exoribonuclease activity. Taken together, we demonstrate that YB-1 tightly controls intracellular IL-6 mRNA levels by binding and exporting cytosolic IL-6 mRNA to the extracellular fluid, but does not act as an exoribonuclease. 3 Functional Role of YB-1 in Controlling Intracellular IL-6 mRNA Levels Intracellular YB-1 is essential for IL-6 mRNA stability in dendritic cells Because YB-1 is involved in regulating transcription, RNA processing, and mRNA stabilization, we next investigated whether intracellular YB-1 controls IL-6 mRNA metabolism in dendritic cells. To examine the effects of YB-1 on IL-6 mRNA transcription and splicing, dendritic cells expressing YB-1 RNAi were stim