viral symptom development in the course of acute infection [36]. Pasin et al. (2020) demonstrated

viral symptom development in the course of acute infection [36]. Pasin et al. (2020) demonstrated that ABA can handle RNA biology, including RNA stability, turnover, maturation, and translation, in viral suppressor transgenic plants [5]. Compared with all the HTP NGS, LTP NGS represents a much more economical method for transcriptome studies as a result of the pooling of additional sample libraries. In this study, the DEGs identified using the LTP profiles could reflect the essence in the HTP profiles because we performed sequencing at only one-tenth on the sequencing depth utilized within the HTP evaluation. The critical genes correlated with drought pressure, cold stress, senescence, andViruses 2021, 13,24 ofthe RNA silencing pathway had been accurately identified using the LTP dataset. Seventy-five network genes had been identified with each the HTP and the LTP profiles, and these network genes included gene encoding PTGS elements (i.e., AGO1 and AGO2) and miRNA targets (i.e., SPL3, SPL13A; CSD1, CSD2, CCS, and TOE2). These final results imply that the LTP technique features a specific amount of accuracy with respect to transcriptome evaluation. Nevertheless, the Pearson correlation coefficients were not 100 identical in between the HTP and LTP profiles. A low number of genes might be excluded in the LTP analysis, which would bring about differences in matrix genes and thus differences in the Pearson correlation coefficients in between the HTP and LTP profiles. Therefore, we advocate that the LTP approach is often applied in preliminary or main studies to determine crucial genes and to identify a backbone network prior to a far more in-depth HTP approach is regarded as. 5. Conclusions By using comparative networks in the HTP RNA-Seq profiles, it revealed that P1/HC-ProTu can trigger many phytohormone-mediated strain responses, specifically the regulatory signaling pathways controlled by ABA/Ca2+ . Upon stimulation by P1/HCProTu , expression alterations of genes involved in a variety of cellular processes, which include drought and cold tension responses, stomatal dynamics, senescence, cell death, and chlorophyll degradation, had been linked to viral suppressor induced responses. The P1/HC-ProTu -mediated ABA disturbances by way of PTGS suppression may alter these gene expressions. Using a LTP NGS method, we can simulate the HTP network for studying the P1/HC-ProTu -mediated PTGS suppression.Author Contributions: Conceptualization, S.-S.L.; methodology, Y.-L.H., Y.-H.C., W.-L.W. and Z.-J.P.; investigation, Y.-H.C., H.-P.W., Q.-W.S., T.H.P., C.-K.H. and Z.-J.P.; information curation, S.-S.L. and Z.-J.P.; writing–original draft preparation, S.-S.L. and Z.-J.P.; writing–review and editing, S.-S.L. and Z.-J.P.; supervision, S.-S.L.; visualization, Z.-J.P. All authors have study and agreed for the published version of your manuscript. Funding: This investigation was funded by the Ministry of Science and Technology (MOST) of Taiwan (MOST 108-2311-B-018-MY3), Joint Center for Instruments and Researches, College of Bioresources and Agriculture, NTU (110KKZT69A), and CH Biotech Organization (Caspase 7 Activator MedChemExpress 110HT654004). Institutional Evaluation Board Statement: Not applicable. Informed Consent Statement: Not applicable. Information Availability Statement: The information presented in this study are obtainable on request from the corresponding author. Acknowledgments: We acknowledge the deep sequencing CDK1 Activator custom synthesis technologies in the Higher Throughput Sequencing Core of Academia Sinica. We also thank the service of LC/MS evaluation by the Metabolomic Core Facility, in the Agricultural Biotechnology Researc