He onset of a variety of ageassociated illnesses and chemotherapy induced premature ageing. Methods: As

He onset of a variety of ageassociated illnesses and chemotherapy induced premature ageing. Methods: As a way to fully grasp how senescent cells that accumulate within organisms with age negatively effect on organ and tissue function, we have characterized senescent cell derived extracellular vesicles (EVs) and their miRNA cargo and their functional part in the context of cellular and organismal ageing, in particular on how EV derived miRNAs influence differentiation and proliferation of skin keratinocytes and mesenchymal stem cells. Outcomes: We identified EVs and circulating miRNAs as bona fide members of the senescence related secretory phenotype (SASP) which can be transferred from senescent cells to their microenvironment or even the systemic atmosphere. Upon uptake, recipient cells alter their behaviour, like changes in osteogenic differentiation of mesenchymal stem cells, in wound healing of skin keratinocytes or apoptotic behaviour of skin fibroblasts. Particularly within the context of osteogenic differentiation, we had been additional capable to show that circulating miRNAs are prognostic biomarkers of osteoporotic fracture danger. Summary/Conclusion: In summary, we present evidence of the importance of certain miRNAs and highlight their prospective use as biomarkers of ageing and age-associated illnesses, or even as therapeutic tools and targets to prevent age-associated diseases. Funding: This study was funded by Christian Doppler Gesellschaft, FWF, EU FP7 SYBIL, EU FP7 Frailomic.PS06.13 = OWP1.Prostate cancer-derived extracellular vesicles facilitate osteoclast fusion and differentiation via enhancing filopodia formation in osteoclast precursorsSaturday, 05 MayPS07: EVs in Tumor Metastasis Chairs: Takahiro Ochiya; Carla Oliveira Location: Exhibit Hall 17:158:PS07.Extracellular vesicles in an in vivo technique for COX Inhibitor list macrophage migration Karen Linnemannstoens; Leonie Witte; Julia Christina Gross University Health-related Center Goettingen, Goettingen, GermanyBackground: Cell migration is a polarized cellular method in which the protruding major edge opposes a retracting trailing edge. EVs handle directionally persistent cell migration by making an autocrine local gradient. This requires polarized delivery of MVBs for the plasma membrane and subsequent polarized secretion. Although the majority of the research in cell culture concentrate on migratory phenotypes, EV studies in developmental/physiological signalling have largely been performed in polarized epithelia like imaginal discs. The question is whether exactly the same cellular machineries direct the selective sorting of cargo into distinct vesicles which are then secreted apically vs. basally or from the major vs. trailing edge respectively. Solutions: To understand this complicated course of action inside a multicellular organism, we study EV biogenesis and polarized secretion inside the model of migrating Drosophila pupal haemocytes, which are elements of the haemolymph and constitute blood cells and macrophages in flies. Whereas isolation of EVs from cell culture supernatants and human serum are established, neither the presence nor H3 Receptor Antagonist Compound function of secreted EVs in haemolymph has been studied so far. We established a method to purify EVs from haemolymph by differential centrifugation and analysed the resulting pellets by several techniques. We generated quite a few EV reporter fly lines expressing fluorescently labelled haemocytes and EV marker. These lines permit to visualize both haemocytes and vesicles inside the cells and characterize their.