Lse S - t) and Sonneborn Stiftelse.Background: Infection with human papilloma virus (HPV) is definitely

Lse S – t) and Sonneborn Stiftelse.Background: Infection with human papilloma virus (HPV) is definitely an important pathological element in head and neck squamous cell carcinoma (HNSCC). Two categories of HNSCC could be distinguished in terms of HPV status: HPV(+) and HPV(-). HNSCCs differ from every single other in respect to their biology and response to therapy. Exosomes are developed by all living cells and mediate intercellular communication. Their protein profiles resemble these of parent cells. Exosomes interact with and reprogram functions of human immune cells. The aim of this study was to examine protein profiles of tumour cell-derived exosomes by mass spectrometry for the presence of proteins which could interact with immune cells and modulate their functions. Solutions: We studied protein profiles of exosomes released by cells of three HNSCC HPV(+) cell lines: SCC-2, SCC-47, SCC-90 and two HNSCC HPV(-) cell lines: PCI-13 and PCI-30. Exosomes have been isolated from tumour cell supernatants by min-size exclusion chromatography (mini-SEC). The isolated exosomes had been assessed for: (1) KIR2DS4 Proteins Biological Activity morphology and size by transmission electron microscopy; (two) quantity of vesicles by q-Nano; and (three) the protein content material. Molecular profiles have been determined applying high-resolution tandem mass spectrometry (LC-MS/MS) technique. The outcomes had been confirmed employing on-bead flow cytometry method. Results: Exosomes originating from HPV(+) and HPV(-) cancer cells had the exact same size (3050 nm) and morphology. Having said that, only HPV(+) exosomes contained the following proteins: E6/E7, Rb and survivin, whilst HPV(-) exosomes have been adverse for cyclin D1 and had low levels of p53. Application of high-resolution mass spectrometry enabled detection of CD47 and CD276 receptor proteins detected only in exosomes originating from HPV(+) cells. Summary/Conclusion: As both of these proteins play essential roles in exosome interactions with immune cells, the data recommend that HPV(+) cancers modulate the host immune program differently than HPV(-) cancers. Funding: The research was financed in element by the Polish National Science Centre project no. [2013/11/B/NZ7/01512].LBT03.Proteomic analysis of exosomes released from irradiated head and neck cancer cells Agata Abramowicz1; Mateusz Smolarz1; Lukasz Marczak2; Piotr Widlak1; Monika PietrowskaMaria Sklodowska-Curie Institute – Oncology Center, Gliwice Branch, Gliwice, Poland; 2Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, PolandLBT03.Proteome of exosomes released by HPV(+) and HPV(-) head and neck cancer cells Monika Pietrowska1; Lukasz Marczak2; Agata Abramowicz1; Marta Gawin1; Sonja Funk3; Priyanka Sharma4; Piotr Widlak1; Theresa L. WhitesideBackground: Head and neck squamous cell carcinoma is the sixth most typical cancer worldwide using a poor prognosis. Deeper Jagged-2 Proteins Formulation understanding of resistance mechanisms induced in cancer cells for the duration of radiotherapy may well contribute to improvement of HNSCC curability. We believe that exosomes reported as significant players in intercellular communication may play a important role in response to radiation and other genotoxic agents utilised in cancer treatment. Approaches: UM-SCC6 cells had been irradiated with doses of 2, four, and eight Gy and cell culture supernatant was collected after 24 h. ExosomeThursday, 03 Maysamples had been purified by differential centrifugation and filtration (0.22 ), then supernatant was concentrated and finally separated with SEC. Collected fractions had been assessed by immunodetection of tetraspanin markers,.