Trosprayanalysis: 123.0553 m/z (mass-to-charge ratio) for NAM; 124.0393 m/z for NATrosprayanalysis: 123.0553 m/z (mass-to-charge ratio)

Trosprayanalysis: 123.0553 m/z (mass-to-charge ratio) for NAM; 124.0393 m/z for NA
Trosprayanalysis: 123.0553 m/z (mass-to-charge ratio) for NAM; 124.0393 m/z for NA; 664.1169 m/z for NAD; 666.1169 m/z for NADH; 335.0644 m/z for NMN; 336.0484 m/z for NAMN; 665.1010 m/z for NAAD.J. Clin. Med. 2021, ten, 5208 five of 12 Table two. Percentage gradient distribution of mobile phases throughout chromatographic separation of LC-MS/MS for nucleotide-related metabolites (phase A–10 mM of ammonium acetate with 0.1 FA in water, phase B–10 mM ammonium acetate with 0.1 FA in methanol).ionization (ES) sort, operating in good ion mode. The samples had been ionized B [ ] the Time [min] Phase A [ ] Phase making use of ESI. The source parameters were optimized to obtain the highest sensitivity for the tested 0 99 1 compounds. The electrode voltage was 4500 V. The other parameters with the ion source C; desolvation temperature: 350 C. 100 were set as 2.7 follows: ion source temperature:0 110 5.7 0 one hundred Nitrogen was used as a nebulizing and drying gas. Quanlynx application (Waters) was applied to five.71 and procedure the data. The following ions have been utilized for quantitative collect 99 1 evaluation: 123.0553 m/z (mass-to-charge ratio) for99 NAM; 124.0393 m/z for NA; 664.1169 m/z for 13 1 NAD; 666.1169 m/z for NADH; 335.0644 m/z for NMN; 336.0484 m/z for NAMN; 665.1010 m/z for NAAD. The chromatograms of NAD metabolites ofof sample from representative patient are metabolites sample from representative patient will be the chromatograms of NAD shown inin Figure 1. Figure 1. shown2018_02_06_JP_N7K_WZ13.53 102.1: TOF MS ES 665.101 0.PF-06454589 manufacturer 0500Da 3.74e0 0.20 0.40 2018_02_06_JP_N7K_WZ10.0.1.1.1.1.1.2.2.two.2.two.two.95 123.3.3.3.3.3.four.4.4.4.four.five.5.5.five.five.six.six.6.six.six.80 1: TOF MS ES 336.048 0.0500Da two.16e2.72 102.13 three.54 102.0 0.20 0.40 2018_02_06_JP_N7K_WZ1 100 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 two.20 two.40 2.60 two.two.94 123.3.three.three.three.three.four.four.4.4.four.5.5.5.five.five.six.six.6.6.6.80 1: TOF MS ES 335.064 0.0500Da 1.54e0 0.20 0.40 2018_02_06_JP_N7K_WZ10.0.1.1.1.1.1.two.two.two.2.two.three.three.three.three.51 102.13 three.49 102.3.3.four.four.4.four.4.five.5.five.5.five.six.six.six.six.6.80 1: TOF MS ES 666.133 0.0500Da 9.52e3.33 three.42 102.13 102.three.58 102.13 3.71 102.0 0.20 0.40 2018_02_06_JP_N7K_WZ1 100 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 two.20 two.40 2.60 2.80 3.00 three.20 three.3.53 102.6.07 102.six.58 149.six.80 102.3.3.4.four.four.four.four.five.five.five.five.5.six.six.six.six.six.80 1: TOF MS ES 664.117 0.0500Da 9.74e0 0.20 0.40 2018_02_06_JP_N7K_WZ10.0.1.1.1.1.1.two.2.two.2.2.three.three.3.three.three.three.80 124.4.four.four.four.four.5.5.five.5.5.6.six.six.6.six.80 1: TOF MS ES 124.039 0.0500Da three.49e4.29 123.0 0.20 0.40 2018_02_06_JP_N7K_WZ1 one hundred 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 two.20 2.40 2.60 2.80 3.00 3.20 three.40 three.60 three.80 4.00 4.four.29 123.four.four.4.5.5.5.five.five.six.6.6.6.6.80 1: TOF MS ES 123.055 0.0500Da 6.67e2.95 123.0 0.20 0.40 2018_02_06_JP_N7K_WZ14.29 123.0.0.1.1.1.1.1.two.two.two.2.two.three.3.3.3.three.4.4.four.4.4.five.5.5.5.5.6.6.6.six.57 149.six.6.80 1: TOF MS ES TIC two.47e6.85 102.0.17 102.three.82 124.04 2.95 123.four.46 153.5.93 149.0 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 two.00 two.20 2.40 2.60 2.80 3.00 3.20 3.40 three.60 3.80 four.00 four.20 four.40 four.60 four.80 5.00 five.20 five.40 five.60 5.80 six.00 six.20 six.40 6.60 six.TimeFigure 1. Total Total ionic existing (TIC) chromatogram for patient exemplified sample. Information representsingle ion present chroFigure 1. ionic Sutezolid Bacterial,Antibiotic current (TIC) chromatogram for patient exemplified sample. Data represent single ion present matograms for specific metabolites (NAAD, NAMN, NMN, NADH, NAD, NA and NAM, from the leading) from with their chromatograms for particular metabolites (NAAD, NAMN, NMN, NADH, NAD, NA and NAM, from the top rated) from with retention times on horizon.